In the specialized field of i Regulatory Status: RUO ; Other Names: MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb,OVA257-264( Read more mmunology research, the OVA257-264 peptide—commonly referred to by its amino acid sequence SIINFEKL—stands as a cornerstone for experimental models. As someone deeply engaged in the study of biological sequences, I have found that this octameric peptide, derived from chicken ovalbumin, is indispensable for understanding the dynamics of antigen presentation and cellular responses.
Defining the OVA257-264 Peptide
The OVA257-264 peptide is widely recognized as a class I (Kb)-restricted peptide epitope of OVA. It functions as an ovalbumin-derived peptide that is processed and presented by the H-2Kb major histocompatibility complex (MHC) molecule. With a molecular weight of approximately 963.1 g/mol and the chemical formula C45H74N10O13, it serves as the gold standard for researchers aiming to observe interaction specificity within the MHC system.
Practical Applications in Laboratory Research
My experience with this molecule highlights why it remains a critical tool. Because OVA 257-264 peptides can be used to detect a stron OVA (257-264) Peptide Fragment g CD8+ cytolytic T-cell response, they are frequent OVA (257-264) is a class I (Kb)-restricted peptide epitope of ovalbumin (OVA), presented by the class I major histocompatibility complex (MHC) molecule, H-2Kb.Read more ly employed in assays designed to track the specificity of T-cell receptors.
When evaluating these research tools, practitioners often consider several variations:
* FAM-OVA (257-264) is a biological active peptide: The inclusion of a FAM label allows for fluorescent tracking, which is essential for visualizing localization within a sample.
* OVA-T4 Peptide: OVA (257-264) Q4 Variant - AnaSpec A known variant (SIITFEKL) often used alongside the standard sequence to investigate binding affinities and receptor sensitivity.
* Scrambled Versions (FILKSINE): These act as vital negative controls in experimental design, ensuring that the observed cellular activity is specific to the correct sequence.
Research Efficacy and Methodology
In my work, I have observed that OVA 257-264 is also used to test new adjuvant in immunotherapeutic vaccine development. The ability of this sequence to form stable hydrogels provides unique opportunities for sustained delivery studies. Furthermore, the use of 25-D1.16 monoclonal antibody has proven highly effective for researchers to identify the SIINFEKL (OVA 257-264) peptide when it is specifically bound to the MHC class I molecule.
Whether you are looking for high-purity OVA (257-264) for precise quantitative analysis or using it within an OVA257-264 (SIINFEKL)-based assay, the consistency of this antigenic peptide is paramount. It is an OVA (257-264 tool that continues to bridge the gap between the SIINFEKL-10mg [5887] - 0.00 oretical modeling and observable data.
Key Considerations for Experimental Success
When sourcing this materia OVA257-264 (SIINFEKL) peptide bound to H-2Kb l, the purity of the OVA peptide (257-264) is the most significant factor. Low-quality synthesis can lead to inconsistent binding with the H-2Kb molecule, which would skew results in any CD8+ cytolytic T-cell response study. Always verify the CAS number (138831-86-4) to ensure you are working with the authentic chicken ovalbumin peptide.
By focusing on the interaction between the OVA257 and 264 residues within the MHC groove, researchers can continue to refine their understanding of immune recognition. As an OVA1 peptide, it remains the primary reference point for those exploring the mechanisms of antigen presentation. Through careful selection of these high-quality research materials, we can achieve more reproducible and meaningful results in our laboratory settings.